In the present study in-vitro seed germination protocol using various pre-sowing treatments for Aconitum heterophyllum Wall intended for its ex-situ conservation was optimized. Temperature requirement for seed germination in this species was optimized under controlled conditions which revealed that low temperature of 15°C (p<1.0%) was obligatory. Two in-vitro presowing treatments: PST-1 (pre-treated seeds kept at normal room temperature) and PST-2 (pre-treated seeds kept at 15°C) were also studied. Highest seed germination was recorded with PST-2 (p<0.1%) with 0.5 mg L-1 IAA (97.17%) as compared to PST-1 (p<1.0%). As seed embryo in A. heterophyllum Wall is small, it requires low temperature to grow and also GA3 (100 and 200 μM) could not improve the seed germination even at low temperature; so it may be assumed that it exhibit deep complex morphophysiological dormancy (MPD).
Nidhi Srivastava, Vikas Sharma, Anoop K. Dobriyal, Barkha Kamal, Sanjay Gupta and Vikas S. Jadon, 2011. Influence of Pre-Sowing Treatments on in vitro Seed Germination of Ativisha (Aconitum heterophyllum Wall) of Uttarakhand. Biotechnology, 10: 215-219.