The genus Allium, consists of hundreds of medicinal plant species, is one of the most imperative sources of life supporting drugs. The in vitrobiotechnological interventions are vital to choose, multiply, store up and improve the major Allium sp. In vitro culture of Allium has performed an incredibly crucial role in accelerated growth of several species with desirable traits and production of healthy and disinfectant propagules as well as paved the way towards cultivar improvement. During the last quite a few years, several moves have been made forin vitro propagation of Allium. In vitro regeneration via direct and indirect organogenesis using different explants and plant growth regulator formulations has been comprehensively covered in the literature. Recent challenges for establishment of protocols for genetic transformation have gained preference in the recent past reports. This review article comprehensively describes the exploitation of biotechnology forin vitro regeneration and genetic transformation for enhancement of the genus Allium.