Subscribe Now Subscribe Today
Science Alert Home Journals at Science Alert For Authors For Subscribers Contact Us
   
Research Journal of Phytochemistry
  Year: 2011 | Volume: 5 | Issue: 2 | Page No.: 80-88
DOI: 10.3923/rjphyto.2011.80.88
Antioxidant Activity, Lipoxygenase Inhibitory Effect and Polyphenolic Compounds from Calotropis procera (Ait.) R. Br
Mohammad Ahmad Khasawneh, Hanan Mohamed Elwy, Nael Mohamed Fawzi, Alaaeldin Ahmed Hamza, Abdul Raheem Chevidenkandy and Ahmed H. Hassan

Abstract:
The purpose for this study was to evaluate the biological, antioxidant, anti-inflammatory and anti-cancer properties of Calotropis procera alcoholic extract and its various solvent subfractions. The total antioxidant property was estimated by the Ferric Reducing Antioxidant Power (FRAP), 1,1-diphenyl-2-picrylhydrazyl (DPPH·), 2, 2-azino-bis (3-ethylbenzothiazoline-6-sulfonate) (ABTS·+) and β-carotene bleaching tests. Among the ethanol extract and three fractions, the ethyl acetate fraction showed the highest phenolic content (108.26 mg gallic acid g-1) and the best antioxidant activity. The ascorbic acid equivalent antioxidant capacities of the ethyl acetate fraction were 0.26, 0.53, 0.17 mmol g-1 in FRAP, ABTS and DPPH assays, respectively. This study verified that the ethanol extract and ethyl acetate fraction from Calotropis procera have strong antioxidant activity that were correlated their high level of phenolic content. Furthermore, the same extract showed appreciable anti-inflammatory via lipoxygenase inhibitory activity (LOX), IC50 values ranged from 7.12-52.68 μg mL-1. Moreover, ethyl acetate fraction showed the strongest cytotoxic effect (IC50 = 10.6 μg mL-1) against MCF-7 breast cancer cell line.
 [Fulltext PDF]   [Fulltext HTML]   [XML: Abstract + References]   [References]   [View Citation]  [Report Citation]
 RELATED ARTICLES:
  •    Antioxidant and Antimicrobial Activity of Araucaria cookii and Brassaia actinophyla
  •    In vitro Antioxidant Activity, Phytochemical Screening, Cytotoxicity and Total Phenolic Content in Extracts of Caesalpinia pulcherrima (Caesalpiniaceae) Pods
  •    HPLC/DAD Profile and Determination of Total Phenolics, Flavonoids, Tannins and Alkaloids Contents of Scutia buxifolia Reissek Stem Bark
  •    Evaluation of Antioxidant, Anti-inflammatory and Analgesic Potential of the Luffa acutangula Roxb. Var. amara
  •    Hepatoprotective and Antioxidant Activity of Root Bark of Calotropis procera R.Br (Asclepediaceae)
  •    Evaluation of Antifungal Activity and Phytochemical Analysis of Leaves, Roots and Stem Barks Extracts of Calotropis procera (Asclepiadaceae)
  •    Analysis of Essential Oil Constituents in Hydro-Distillates of Calotropis procera (Ait.) R.Br
  •    Medicinal Potential of Poisonous Plants of Tehsil Kahuta from District Rawalpindi, Pakistan
How to cite this article:

Mohammad Ahmad Khasawneh, Hanan Mohamed Elwy, Nael Mohamed Fawzi, Alaaeldin Ahmed Hamza, Abdul Raheem Chevidenkandy and Ahmed H. Hassan, 2011. Antioxidant Activity, Lipoxygenase Inhibitory Effect and Polyphenolic Compounds from Calotropis procera (Ait.) R. Br. Research Journal of Phytochemistry, 5: 80-88.

DOI: 10.3923/rjphyto.2011.80.88

URL: http://scialert.net/abstract/?doi=rjphyto.2011.80.88

 
COMMENT ON THIS PAPER
.
 
 
 
 

 

 
 
 
 
 
 
 
 
 

 
 
 
 
 
 
 

                 home       |       journals        |       for authors       |       for subscribers       |       asci
          © Science Alert. All Rights Reserved