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Research Journal of Mutagenesis
  Year: 2011 | Volume: 1 | Issue: 1 | Page No.: 1-9
DOI: 10.3923/rjmutag.2011.1.9
Cytotoxic and Genotoxic Effects of Cassava Effluents using the Allium cepa Assay
D.I. Olorunfemi, G.E. Okoloko, A.A. Bakare and A. Akinboro

Abstract:
Agricultural wastes generated from pilled cassava tubers contribute significantly to pollution of the environment. In this study, we investigated the potential genotoxic effects of the effluents obtained from the processing of cassava tubers into three popular Nigerian cassava meals; garri, lafun and akpu using the modified Allium cepa assay. A series of 10 onion bulbs were cultivated in 0.001, 0.01, 0.1, 1.0 and 10% concentrations (effluents, v/v) of each of the test samples (garri, lafun and akpu). At 48 h, root tips from the treated bulbs were processed for cytological studies by the acetocarmine or orcein-orcein squash technique. At 72 h, their cytotoxic effects on the onion root tips showed strong growth retardation in high concentrations of all the effluents with EC50 values of 1.5, 2.5 and 3.5% for garri, lafun and akpu effluents, respectively while total phytotoxic effects was induced by the undiluted effluents. The physico-chemical properties of the effluents revealed the presence of significant amounts of cyanide and heavy metals. Root length inhibition, breakages and malformations were characterized by the presence of crochet hooks and c-tumors at low effluent concentrations (10-1, 10-2 and 10-3%). There was a rapid decrease in mitotic index with increasing effluent concentration. Effluents-induced chromosome aberrations in the root tip cells were statistically significant (p<0.05). The present findings indicate that the substances contained in the cassava effluents may be toxic to living organisms and may pollute the environment.
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How to cite this article:

D.I. Olorunfemi, G.E. Okoloko, A.A. Bakare and A. Akinboro, 2011. Cytotoxic and Genotoxic Effects of Cassava Effluents using the Allium cepa Assay. Research Journal of Mutagenesis, 1: 1-9.

DOI: 10.3923/rjmutag.2011.1.9

URL: http://scialert.net/abstract/?doi=rjmutag.2011.1.9

COMMENTS
01 June, 2010
DR. AWADHESH KUMAR YADAV:
present article is best by scientific work and knowledg and have proper approach to mutation causal elements.
27 June, 2011
Tyya:

Now I know who the braniy one is, I’ll keep looking for your posts.

29 June, 2011
DR. AWADHESH KUMAR YADAV:

TAYYA , I M ALSO DOING WORK ON MUTATAGENESIS N CYTOLOGY OF MONOCOT DICOT, AT DEP. OF BOTANY, UNIVERSITY OF LUCKNOW AS A RESEARCHER IN INDIA.

 
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