In the present study the polyphenolic content, antibacterial activity and antioxidant activity of Trigonella foenum graecum leaves in chloroform, hexane, methanol, ethanol and water extracts were carried out. Total phenolic content was estimated by Folin Ciocalteau method while the flavonoid content was determined using aluminum chloride method. Estimation of total phenolic and flavonoid contents revealed that, the ethanol extract had the highest phenolic and flavonoid contents of 4.9 and 0.47 mg g-1, respectively. The antibacterial activity of various extracts was screened by disc diffusion method and ethanol extract was found to be more potent. The Minimum Inhibitory Concentration (MIC) of ethanol extract determined by broth dilution method showed a MIC value of 1 mg mL-1 for Staphylococcus aureus and Pseudomonas aeruginosa. The antioxidant activities of the leaves were evaluated by various in vitro assays like ferrous reducing antioxidant assay, total reducing power, 1, 1-Diphenyl-2-Picryl Hydrazyl (DPPH) scavenging activity, lipid peroxidation inhibitory activity and superoxide scavenging activity. Among the solvent extracts tested for antioxidant activities, ethanol extract had the highest activity compared to other solvent extracts. It had a total antioxidant activity of 47 μM Fe (II) g-1 and an Inhibitory Concentration (IC50) value of 0.3 and 0.7 mg mL-1 for lipid peroxidation inhibitory activity and DPPH scavenging activity, respectively. These results suggested that ethanol extract of fenugreek leaf is not only an important source for antibacterial components but also a potential source of phenolic antioxidants.
Ramya Premanath, J. Sudisha, N. Lakshmi Devi and S.M. Aradhya, 2011. Antibacterial and Anti-oxidant Activities of Fenugreek (Trigonella foenum graecum L.) Leaves. Research Journal of Medicinal Plants, 5: 695-705.