Adult male Wistar rats were fed with arginine (ARG) (60 mg kg¯1 b.wt.), glutamate (GLU) (90 mg kg¯1 b.wt.), monosodium glutamate (MSG) (15 mg kg¯1 b.wt.), ARG+GLU (60:90 mg kg¯1 b.wt.) or ARG+MSG (60:15 mg kg¯1 b.wt.). The feeding was by oral intubation and was daily for 28 days. The aim was to investigate possible alterations in the serum biochemistry and liver histology induced by ARG, GLU or MSG either alone or in such combinations as ARG+GLU or ARG+MSG. After 28 days oral treatment, rats treated with ARG, GLU or MSG alone significantly (p<0.05) increased the serum alkaline aminotransferase (ALT) activity, whereas rats co-treated with ARG and either GLU or MSG decreased (p<0.05) the serum ALT activity. In comparison with the control, the other treatment groups elicited a significant quantitative increase in the serum aspartate aminotransferase (AST) activity and the computed AST:ALT ratio but, the observed increase in the ARG-treated group was consistently lower relative to the other groups. Liver sections of rats from the various treatment groups showed varying degrees of focally diffuse (random) hepatocellular necrosis. On the whole, the study suggests that sub-acute daily oral treatment of male Wistar rats with ARG, GLU or MSG alone or ARG together with GLU or MSG could possibly impair their physiological functions as evidenced in the significant alterations in the serum biochemistry and liver histology of this study. The results are considerably significant going by the possible inadvertent abuse of ARG, GLU and MSG alone or together in diets and drugs.
A.C.C. Egbuonu, L.U.S. Ezeanyika, P.M. Ejikeme and O. Obidoa, 2010. Histomorphologic Alterations in the Liver of Male Wistar Rats Treated with L-Arginine Glutamate and Monosodium Glutamate. Research Journal of Environmental Toxicology, 4: 205-213.