In this research, we have
aimed to determine antagonistic effects of various lactic acid bacteria
against Gram (+) and Gram (-) bacteria with a comparison of disc diffusion
and spot-on-lawn method. In spot-on-lawn method, P. aeruginosa
ATCC 27853 was the most sensitive of the tested bacteria, followed by
E. coli ATCC 25927 and P. aeruginosa ATCC 10145. On the
other hand, in disc diffusion assay B. subtilis ATCC 6633, E.
coli O157:H7 and P. aeruginosa ATCC 10145 were the most sensitive
indicator microorganisms. The spot-on-lawn method was suitable for assessing
the antagonistic effect of lactic acid bacteria. In general, inoculum
density had little effect on inhibition.