In this study, launea arborescens (Asteraceae) was selected for phytochemical investigation. The important activity of the methanol extract detected in the biological and chemical screening performed previously and the lack of studies concerning the genus prompted this choice. The aerial parts methanol extract of launea arborescens (Asteraceae) displayed several activities: amongst these, the antifungal activities against Candida albicans and Sacharomyce cereviveae and the antibacterial activity against E. coli, S. aureus, P. aeriginisa and Klebseila entrecocus. The extracts presenting the most interesting activities are then selected for activity-guided phytochemical investigation, in order to identify the active compounds. Moreover, the isolation and characterization of further molecules presenting original chemical structures and a potential therapeutic interest is performed.