Twenty-one monosomic lines of variety Chinese Spring (apically fertile) were crossed with variety Y82187 (apically sterile) in order to identify chromosomes carrying genes for water-stress induced apical sterility. Segregation in a disomic F2 family obtained from a cross between CS and Y82187 was also evaluated under water-stress conditions. The results with the disomic F2 population suggested that 2 genes with complementary interaction control water-stress induced apical sterility. F2 monosomic analysis revealed that chromosome 3A of line Y82187 bred true for apical sterility. Monosomics for chromosomes 3D, 5D and 6D showed additional apically sterile plants in comparison with the disomic F2 population, whereas the data for other monosomic populations did not depart from the predicted 9:7 ratios. F2 progenies for some fertile and some sterile plants from F2 families monosomic for chromosomes 3D, 5D and 6D were evaluated under water-stress conditions in order to provide more information about these families. The results suggested that, in addition to chromosome 3A, chromosome 3D is the most likely chromosome to carry the second locus responsible for water-stress induced apical sterility in variety Y82187.