Subscribe Now Subscribe Today
Science Alert Home Journals at Science Alert For Authors For Subscribers Contact Us
   
Pakistan Journal of Biological Sciences
  Year: 2006 | Volume: 9 | Issue: 7 | Page No.: 1328-1332
DOI: 10.3923/pjbs.2006.1328.1332
Characterization and Optimization of α-amylase Activity of Streptomyces clavifer
Md. Mahfuzul Hoque, Mahmuda Khanam , Md. Arif Shiekh , Nurun Nahar , Mahbubar Rahman Khan and Zahed U.M. Khan

Abstract:
Amongst seventeen previously identified Streptomyces species, S. crystallinus, S. noboritoensis, S. anulatus and S. clavifer were selected for α-amylase activity. All these species were grown in fermentation broth for α-amylase production in orbital shaker under optimum conditions. The fermentation broth cultures were then centrifuged at refrigeration temperature at 1000 rpm and supernatants were collected. The α-amylase from each supernatant was extracted by ammonium sulfate precipitation at 90% saturation and was dialyzed. The enzyme was assayed under standard assay conditions. Streptomyces clavifer was found to have the highest α-amylase activity amongst others. The effect of pH and temperature on α-amylase activity of Streptomyces clavifer was determined. The optimum pH and temperature for enzyme activity was found be 7.0 and 45°C, respectively. The enzyme was found stable at pH 6.0 to 8.0 and temperature upto 55°C. The kinetics of the enzyme was determined under standard conditions. The KM and the Vmax of the enzyme were determined as 4 mg and 0.03 mg sec-1, respectively. α-amylase together with other amylolytic enzymes have tremendous applications in textile industries, confectioneries, pharmaceutical industries and other food industries as hydrolyzing bioagents.
 [Fulltext PDF]   [Fulltext HTML]   [XML: Abstract + References]   [References]   [View Citation]  [Report Citation]
How to cite this article:

Md. Mahfuzul Hoque, Mahmuda Khanam , Md. Arif Shiekh , Nurun Nahar , Mahbubar Rahman Khan and Zahed U.M. Khan , 2006. Characterization and Optimization of α-amylase Activity of Streptomyces clavifer. Pakistan Journal of Biological Sciences, 9: 1328-1332.

DOI: 10.3923/pjbs.2006.1328.1332

URL: http://scialert.net/abstract/?doi=pjbs.2006.1328.1332

 
COMMENT ON THIS PAPER
.
 
 
 
 

 

 
 
 
 
 
 
 
 
 

 
 
 
 
 
 
 
 
 
 
 
 
 
 
 

                 home       |       journals        |       for authors       |       for subscribers       |       asci
          © Science Alert. All Rights Reserved