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Pakistan Journal of Biological Sciences
  Year: 2006 | Volume: 9 | Issue: 7 | Page No.: 1231-1241
DOI: 10.3923/pjbs.2006.1231.1241
Cloning and Characterization of New emm Allele of Streprococcus pyogenes Strains Isolated in Kingdom of Saudi Arabia
Magdy M. Mohamed

Abstract:
In the present study, 39 isolates of erythromycin-resistant group A Streptococcus (GAS) isolated in the kingdom of Saudi Arabia from 2003 to 2004 were characterized by using phenotypic and genotypic methods. Most strains (94.9%) had similar or highly related pulsed-field gel electrophoresis profiles to different nine emm types previously documented. In which, type emm1 was the most prevalent M type in KSA representing 20.5% of total isolates, followed by emm3 (15.4%) typing. Two new emm sequence types were identified among GAS strains designated SA15 and SA37. The type SA15 carried two resistant mefA and ermTR genes accounted for 6.3% (1/16) and 50.0% (1/2), respectively. Entire fragment of SA15 was sequenced and expressed in expression vector to be used as a tool for vaccine preparation in this area. This report provides information on new emm sequence types firstly detected in KSA GAS isolates as a vaccine candidate antigen in this geographic area which not extensively surveyed, also it contributes to a better understanding of the local and global dynamics of GAS populations and the epidemiological aspects of GAS infections occurring in KSA.
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How to cite this article:

Magdy M. Mohamed , 2006. Cloning and Characterization of New emm Allele of Streprococcus pyogenes Strains Isolated in Kingdom of Saudi Arabia. Pakistan Journal of Biological Sciences, 9: 1231-1241.

DOI: 10.3923/pjbs.2006.1231.1241

URL: http://scialert.net/abstract/?doi=pjbs.2006.1231.1241

 
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