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Pakistan Journal of Biological Sciences
  Year: 2005 | Volume: 8 | Issue: 7 | Page No.: 1051-1054
DOI: 10.3923/pjbs.2005.1051.1054
Extraction of Chitin and Chitosan from Shrimp (Metapenaeus monoceros) Shell by Chemical Method
Rokshana Naznin

The present study was undertaken to extract chitin and chitosan by chemical method. Several treatments of acid and alkali were taken into consideration to determine effective concentration for yielding optimum output. The shells from Hariana Shrimp (Metapenaeus monoceros) were used as raw material in the experiment. Extraction of chitin and chitosan using different concentration of 0.5, 1.0, 1.5 and 2 N commercial grade sodium hydroxide in deproteinization step and different percentages of 10, 20, 30, 40 and 50% commercial grade HCl acid in demineralization step in laboratory scale were done. The dry sample was immersed in HCl solution for demineralization at ambient temperature for over night. Then the sample was collected, washed and dried. To obtain chitosan, deacetilation was carried out by 50% sodium hydroxide solution over night at 60-70°C. In 1% acetic acid solution chitosan dissolved but chitin was insoluble in this solution. Among all treatments, of this research, 30% HCl and 1.5 N NaOH solution yielded better quality chitin and chitosan. The final products were white in color and insoluble in water.
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How to cite this article:

Rokshana Naznin , 2005. Extraction of Chitin and Chitosan from Shrimp (Metapenaeus monoceros) Shell by Chemical Method. Pakistan Journal of Biological Sciences, 8: 1051-1054.

DOI: 10.3923/pjbs.2005.1051.1054






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