The present study was undertaken to extract chitin and chitosan by
chemical method. Several treatments of acid and alkali were taken into
consideration to determine effective concentration for yielding optimum output.
The shells from Hariana Shrimp (Metapenaeus monoceros) were used as raw
material in the experiment. Extraction of chitin and chitosan using different
concentration of 0.5, 1.0, 1.5 and 2 N commercial grade sodium hydroxide in
deproteinization step and different percentages of 10, 20, 30, 40 and 50%
commercial grade HCl acid in demineralization step in laboratory scale were
done. The dry sample was immersed in HCl solution for demineralization at
ambient temperature for over night. Then the sample was collected, washed and
dried. To obtain chitosan, deacetilation was carried out by 50% sodium hydroxide
solution over night at 60-70°C. In 1% acetic acid solution chitosan dissolved
but chitin was insoluble in this solution. Among all treatments, of this
research, 30% HCl and 1.5 N NaOH solution yielded better quality chitin and
chitosan. The final products were white in color and insoluble in water.