Abstract:
An aerobic, alkaliphilic, xylanolytic bacterium was isolated from
local Soda lake. The strains were isolated on xylan agar medium and screened
by xylanolysis method. The bacterial strains ( StA, StB,
StC and StD) were identified based on morphological, biochemical
and physiological characters. On the basis of these tests, the four strains
were identified as belonging to the genus Bacillus. Alkaline Xylanases
produced by four different strains of Bacillus sp. were also characterized.
The optimal pH and temperature were pH 8.0 for and 50°C
StA and pH 8.5 and 55°C for strain StD
and pH 9.0 and 55°C for strains StB
and StC. Under these conditions the following activities were found
after 10 min in the presence of 0.7% oat spelt xylan; 302, 233, 189 and 207
U ml-1, respectively, for the four strains. The enzymes
were stable at 40-50°C, with 45% of the xylanases
activity remaining after 2 hours for the enzymes of strain StB and
55% for the other four strains. Taking into account the conditions under which
Kraft pulps are bleached during the manufacture of paper, Xylanases from Bacillus
sp. exhibit favorable potential for application to bleaching in the paper
making process.
Narayan Roy and M. Belaluddin , 2004. Production and Characterization of Alkaline Xylanases from Bacillus sp.
Isolated from an Alkaline Soda Lake. Pakistan Journal of Biological Sciences, 7: 777-781.
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