Exogenous sugars, especially sucrose is very essential for providing osmotic environment and nutrition to in vitropollen grain germination. Pollen bursting is frequently observed in artificial medium lacking suitable sucrose concentration. Four sucrose concentrations 10, 20, 30 and 40% were tried for okra pollen germination. At 10% sucrose,majority of pollens brusted; however, at 20% sucrose, 80% ofpollen grains germinated by producing tube lengths in the range of 3000 to 4000 μm. At 30%, the pollen germination% and tube length declined by about more thantwice against 20% sucrose level. At 40% concentrations, pollen grains` germination and tube length reduced
remarkably by giving only500 to 700 μm pollen tubes. Not only tube length retarded at these high concentrations but pollentubes also changed to more thickened and shortened structures.It was therefore observed that 20% sucrose is an appropriate concentration for in vitrookra pollen germination.
Mohammed Jurial Baloch , Abdul Rahim Lakho , Hidayatullah Bhutto and Mohammed Yousuf Solangi , 2001. Impact of Sucrose Concentrations on in vitro Pollen Germination of Okra, Hibiscus esculentus. Pakistan Journal of Biological Sciences, 4: 402-403.
27 December, 2009
H.P.P.PREMATHILAKA: Your finding was very important to me.Thank you very much. I am carrying an experiment on reproductive biology of hibiscus furcatus. your informations are very important to me. if u have any information about hibiscus genus or about okra, please forward me.thank you.