Abstract: Trichoderma reesei QM9414 was co-cultured with Aspergillus terreus SUK-1 for cellulase and reducing sugar production on sugar cane bagasse in solid substrate fermentation. The highest cellulase activity (FPase 0.68 IU/ml), β-glucosidase activity (0.08 IU/ml) and reducing sugar concentration (1.4 mg/ml) were obtained in mixed cultures using inoculum ratio of 2:1 (Trichoderma : Aspergillus) compared to other ratios investigated (1:2 and 1:1). Nevertheless, experiments with pure culture of T. reesei was found to be better in terms of the production of FPase (1.4 IU/ml) and reducing sugar concentration (2.3 mg/ml). The addition of A. terreus to T. reesei in cultures of 2:1 inoculum ratio increases the production of β-glucosidase (the highest activity of 0.08 IU/ml was detected in cultures of 2:1 ratio as well as in pure cultures of A. terreus). Nevertheless, this enhanced β-glucosidase activity in mix culture could not result in enhanced substrate utilization and reducing sugar production. It was found that the highest percentage of degradation (25%) was achieved when pure cultures of T. reesei was employed followed by when pure cultures of A. terreus was employed (22%) and when mix cultures of 2:1 inoculum ratio was employed (22%). This suggests that synergism of β-glucosidase and cellulase to enhance substrate degradation was better affected using pure culture than mixed culture in solid substrate fermentation of sugar cane bagasse.
Wan Mohtar Wan Yusoff, Muhannad I. Massadeh , Othman Omar and Jalil Kader , 2000. Sugar Cane Bagasse Degradation by Mixed Culture of T. reesei and A. terreus in Solid Substrate Fermentation. Pakistan Journal of Biological Sciences, 3: 1758-1761.