Three wheat genotypes i.e chakwal-86, NR-58 and Inqilab-91 were tested. Chakwal-86 appeared to be the most responsive genotype to callus induction followed by NR-58 and Inqilab-91. It also produced significantly higher amount of callus compared to other genotypes. Callus induction frequencies varied from 66.65 to 100 percent in Chakwal 86, 49 to 75.80 % in Inqilab-91 and 60.90 to 75.50 % NR-58 on various levels of 2,4-D (2,4-dichlorophenoxy Acetic Acid). However, medium containing 2mg of 2,4-D was found to be optimum for callus induction. Regeneration frequency of Chakwal-86 was 33.33 % on the medium containing 0.1mg of IAA (Indole Acetic Acid) and 2.5 mg of BAP (6-Benzyle Amino Purine). While NR-58 and Inqilab-91 showed regeneration percentage of 40 and 25 % respectively on medium having 0.1 mg of IAA and 0.5mg of BAP. Regenerated plants were transferred to free living conditions. Regenerated plants were evaluated for plant height, maturity and seed set. Regenerated plants showed favourable significant differences from control plants at 5 % confidence level for most of the studied traits; such as plant height, days to maturity and etc.
Ihsan Ullah , Hamid Rashid and Azra Quraishi , 2000. Varietal Response of Wheat, Triticum aestivum L. To Tissue Culture and Assessment of Somaclonal Variation. Pakistan Journal of Biological Sciences, 3: 1598-1600.