Gene overlap between the sporophytic and gametophytic phases and successful in vitro germination of cotton pollen grains have motivated plant breeders that if more reliable and consistent protocols of in vitro pollen germination become available, they could impose certain stresses like heat, salinity and others and selection could be made accordingly in controlled laboratory conditions instead of time consuming and expensive field conditions. Our studies were aimed to utilize two methods of in vitro germination in an artificial medium. One was a droplet method and another was cellophane paper method. The difference in percent of pollen germination and pollen tube length were obvious where droplet method gave higher % of pollen tubes also longer tube length as compared to cellophane paper method. The pollen busting in droplet was also very high and very uncommon on cellophane paper. These results thus suggest that when both the methods are made consistent and reliable, the percent of pollen germination and tube length may be used as criterion of selection for different stress conditions.
Mohammad Jurial Baloch , Abdul Rahim Lakho , Hidayatullah Bhutto and Rahmatullah Rind , 2000. In vitro Germination of Cotton Pollen Grains Using two Different Approaches . Pakistan Journal of Biological Sciences, 3: 1591-1592.