pH and temperature conditions do effect bacterial growth and phage infectivity and development. Bacteria grew better in media at pH 7.0 as compared to other tested pH. Most of these phages showed earlier lysis of host bacteria in cultures at pH 6.5 and maximum phage titre was recorded at pH 7.0. In case of temperature bacteria showed better growth rate at 37°C as compared to 32 and 42°C respectively.
Hamad Siddiqui , Tariq Masud and Muhammad Arif , 2000. Effect of Incubation Temperature and pH on Lactococcus lactis ssp Diacetylactis and Their Virulent Phages . Pakistan Journal of Biological Sciences, 3: 1588-1590.