The objective of this study was to mointer the metabolic disturbance in rats one hour after intoxication with one oral dose of ethanol. Rats orally administered with 10 g ethanol/kg body weight on 24 h empty stomach and sacrificed one hour later. Intoxicated rats recorded significant increase in blood alcohol level (alcohol dehydrogenase; ADH), aspartate (AST) and alanine (ALT) aminotransferase, lactate dehydrogenase (LDH) and gamma glutamyl transferase (GGT) enzyme activities. Carbohydrate deficient transferrin (CDT) level as a new marker for detection early independent ethanol intoxication recorded significant increase. Serum transferrin content was evaluated to avoid the false positive results of CDT. In conclusion, AST, ALT, LDH and GGT may be used as biomarkers for early diagnosis of ethanol toxicity. CDT plays a promise role for early diagnosis. CDT and the other biochemical tests especially GGT are recommended to be detected in parallel to improve the performance of either marker used alone.