A mesophilic marine bacterial strain (strain osh08T) that produced eicosapentaenoic acid and hentriacontanonaene, was identified by the molecular and ordinary microbiological methods. This strain had the high sequence similarity of its 16S rRNA gene to those of Shewanella basaltis JCM 14937T (EU143361; 99.8%) and Shewanella hafniensis NBRC 100975T (AB205566; 98.4%). But, strain osh08T was phenotypically different from S. basaltis J83T and S. hafniensis NBRC 100975T in that it was able to ferment glucose, produce β-glucosidase and β-galactosidase and utilize D-mannose and unable to utilize malate. However, all three strains used iron(III) and manganese(IV) as terminal electron acceptors under anaerobic conditions. The DNA-DNA relatedness between strain osh08T and S. basaltis J83T and that between strain osh08T and S. hafniensis NBRC 100975T were 4.7% and 11.8%, respectively. The G+C content of strain osh08T was 43.4 mol%. Strain osh08T had, in addition to a polar flagellum, many pilus-like structures around the cell that were not observed in the cells of S. basaltis J83T. Thus strain osh08T was identified as a new species of Shewanella. The name Shewanella oshoroensis sp. nov. was proposed and strain osh08T (=NBRC 107685T) is the type strain.
Shinji Sugihara, Ahmad Iskandar Bin Haji Mohd Taha, Taro Motoigi, Akio Ueno, Satoru Shimizu, Mamiko Sato, Takako Kaneko, Kentaro Watanabe, Isao Yumoto and Hidetoshi Okuyama, 2012. Shewanella oshoroensis sp. nov.: A Mesophilic Eicosapentaenoic Acid and Hentriacontanonaene-producing Bacterium. Research Journal of Microbiology, 7: 131-138.