Subscribe Now Subscribe Today
 
FOLLOW US:     Facebook     Twitter
   
Research Journal of Microbiology
  Year: 2011 | Volume: 6 | Issue: 2 | Page No.: 159-165
DOI: 10.3923/jm.2011.159.165
Frequencies of Salmonella enterica in Growing Pigs in Paraguay
J. Copes, G. Leotta, L. Cardozo, G. Gimenez, L. Nunez, N. Zarate, D. Lopez, N. Weiler, M. Alvarez and K. Suzuki

Abstract:
The objective of this study was to (1) estimate frequencies of Salmonella enterica in growing pigs and (2) investigate farmers’ perception and practices towards pig farming in Central Department, Paraguay. Twelve out of 19 districts in the department were selected and 33 farms with growers in the selected districts were recruited. Questionnaire interviews for each study farm, in combination with faecal sample collections (n = 30 per farm), which were microbiologically examined and visual inspection of pig production facilities were performed between March and November 2009. A questionnaire was designed to obtain basic information of a farm such as the number of pigs owned and some selected farming management practices. Faecal samples were used for Salmonella isolation, using buffered peptone water to have salmonellae pre-enriched, followed by xylose lysine tergitol 4 agar and brilliant green sulfapyridine agar. Suspect colonies were biochemically tested by triple sugar iron agar in combination with lysine iron agar to confirm the identity. The true frequency probability and associated 95% Bayesian credible intervals (95% BCI) were computed via the Gibbs sampler, a Markov chain Monte Carlo technique. Overall, 18% (95% BCI: 8-31%) of the tested 1000 faecal samples were classified as positive for Salmonella enterica. All the study farms had at least one positive sample for Salmonella enterica (frequency range: 3-60%). Apparent prevalence at farm-level was therefore 100% (one-sided 97.5% confidence limit: 89%). Twenty-eight different serovars for Salmonella enterica were found. Based on increase the number of study districts, farms as well as animals in combination with improvement of sampling methods, possible spatial differences and risk factors/indicators should be clarified by further investigations.
 [Fulltext PDF]   [Fulltext HTML]   [XML: Abstract + References]   [References]   [View Citation]  [Report Citation]
 RELATED ARTICLES:
  •    Rapid and Simultaneous Identification of Two Salmonella enterica Serotypes, Enteritidis and Typhimurium from Chicken and Meat Products by Multiplex PCR
  •    Evaluation of Ceftriaxone Releasing from Microspheres Based on Starch Against Salmonella spp.
  •    An Investigation on Contamination of Poultries by Salmonella Species in Zahedan (South-East Iran) During 2004
  •    Antimicrobial Susceptibility Pattern and Characterization of Ciprofloxacin Resistant Salmonella enterica Serovar Typhi Isolates in Kerala, South India
How to cite this article:

J. Copes, G. Leotta, L. Cardozo, G. Gimenez, L. Nunez, N. Zarate, D. Lopez, N. Weiler, M. Alvarez and K. Suzuki, 2011. Frequencies of Salmonella enterica in Growing Pigs in Paraguay. Research Journal of Microbiology, 6: 159-165.

DOI: 10.3923/jm.2011.159.165

URL: http://scialert.net/abstract/?doi=jm.2011.159.165

 
COMMENT ON THIS PAPER
.
 
 
 
 

 

 
 
 
 
 
 
 
 
 

 
 
 
 
 
 
 
 

                 home       |       journals        |       for authors       |       for subscribers       |       asci
          © Science Alert. All Rights Reserved