The effect of Cissus repanda Vahl. plant extract
on herpes simplex virus type 1 and type 2 was investigated in this study.
The cytotoxicity of dichloromethane and methanol extracts of C. repanda
on GMK cells was determined by MTT assay. Non toxic concentrations were
used in the study. Methanol extracts of C. repanda showed higher
anti-HSV efficacy than dichloromethane extracts, by plaque reduction assay,
although, HSV particles were directly inhibited by both extracts. Inhibition
of HSV attachment, penetration and replication were also observed, after
treatment of HSV with C. repanda extracts. The most affected stage
was the attachment period. Moreover, C. repanda extracts inhibited
replication of HSV-2 more than HSV-1.