Abstract:
In order to optimize the enzymatic hydrolysis of POME
solid, the effects of substrate pretreatment using varying concentrations
of sodium hydroxide and sulfuric acid, crude enzyme from both strains
in different ratio and pH reaction were studied. The best experimental
conditions found to degrade POME solids were 12 h incubation time, 0.5%
(v/v) sulfuric acid pretreatment, crude enzymes mixture from Aspergillus
niger EB5 and Trichoderma sp. EB6 (1.75 mL Asp+0.25 mL Tri
with the total cellulase activity equal to 14.76 IU) and incubation pH
at 5.0. Under these conditions, the reducing sugar concentration reached
23 g L-1 with the hydrolysis yield and productivity at 32%
and 1.90 g L-1 h-1, respectively. The bioconversion
of POME solid to reducing sugar by the mixture of crude enzyme from the
strains was relatively higher by almost 2 folds as compared to commercial
cellulase. The results suggested that the crude cellulases mixture from
locally isolated fungi has potential for hydrolyzing the abundant agriculture
residues from the palm oil industry.
Wong Kok Mun, Nor`Aini Abdul Rahman, Suraini Abd-Aziz, Vikineswary Sabaratnam and Mohd Ali Hassan, 2008. Enzymatic Hydrolysis of Palm Oil Mill Effluent Solid Using Mixed Cellulases from Locally Isolated Fungi. Research Journal of Microbiology, 3: 474-481.
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