Experimental studies were carried out to produces L-glutamic acid with Brevibacterium roseum free cells, immobilized B. roseum and co-immobilized B. roseum and Escherichia intermedia type 1, production conditions were optimized with B. roseum free cells. Incubation time 96 h, pH 6.0, temperature 30°C, 150 rpm agitation and biotin 1.0 mg mL-1 were optimum for the maximum yield of glutamic acid. When the nutrient ingredients were optimized the glutamic acid yield increased to 40.5 mg mL-1. Immobilized B.roseum produced 37.2 mg mL-1 glutamic acid. Immobilized conditions like alginate concentration, calcium ion concentration, storage period of beads and initial cell loading were optimized. Co-immobilized B. roseum and Escherichia intermedia type-1 produced 39.6 mg mL-1 glutamic acid.
N.M. Yugandhar, Ch. A.I. Raju, P.J. Rao, K. Jaya Raju and D. Sri Rami Reddy, 2007. Production of Glutamic Acid Using Brevibacterium roseum with Free and Immobilized Cells. Research Journal of Microbiology, 2: 584-589.