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Journal of Biological Sciences
  Year: 2014 | Volume: 14 | Issue: 2 | Page No.: 124-133
DOI: 10.3923/jbs.2014.124.133
Incidence of Mycobacterium Avium Subspecies paratuberculosis in Mehsani and Surti Goats of Indian Origin using Multiple Diagnostic Tests
D.B. Barad, B.S. Chandel, A.I. Dadawala, H.C. Chauhan, H.S. Kher, S. Shroff, A.G. Bhagat, S.V. Singh, P.K. Singh, A.V. Singh, J.S. Sohal, S. Gupta, K.K. Chaubey, S. Chakraborty, R. Tiwari, R. Deb and K. Dhama

Abstract:
Mycobacterium avium subspecies paratuberculosis (MAP) is the causative agent of chronic enteric granulomatous inflammation in animals and is known as Johne’s Disease (JD) or Paratuberculosis. JD, being spectral in nature, presents variable bacteriological, immunological and pathological spectra leading to variable efficacy of diagnostic methods at different points of time during the course of infection. The present study aimed to estimate the incidence of MAP in two important breeds of goats (Mehsani and Surti) from South Gujarat region of India by applying conventional, molecular and serological methods. A total 219 goats were screened and categorized into Group-I (123 Mehsani goats), Group-II (76 Surti goats) and Group-III, (20 Non-descript goats). Percent positivity by faecal smear examination, delayed type hypersensitivity (DTH), agar gel immunodiffusion (AGID), IS900 polymerase chain reaction) (PCR) and indigenous enzyme linked immunosorbent assay (ELISA) kit was 9.2 (7/76), 21.9 (27/123), 10.9 (24/219), 12.5 (5/40) and 43.3% (95/219), respectively. Of the 123 goats of Group-I, 27 (21.9%) were positive in DTH test. Of the 5 faecal positive goats which also showed clinical signs, 2 (3.5%) goats died during study were negative by Johnin test. Similar to these findings, sensitivity of Johnin test in goats ranged between 18-30% with least specificity in both preclinical and advanced stage of disease. Of 34 cases of caprine paratuberculosis, 73.5% goats were positive for Johnin test. In the present study, out of the 5 infected goats, 3 (60%) were positive in Johnin test. Rectal pinch smear examination was carried out in 27 DTH positive goats and all smears were negative for the presence of acid fast bacilli. Screening tests (Indigenous ELISA and DTH) showed very high incidence of MAP infection in the goat population. The utility of multiple diagnostic tests is suggested for confirmatory detection and epidemiological diseases investigations of MAP in animals.
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How to cite this article:

D.B. Barad, B.S. Chandel, A.I. Dadawala, H.C. Chauhan, H.S. Kher, S. Shroff, A.G. Bhagat, S.V. Singh, P.K. Singh, A.V. Singh, J.S. Sohal, S. Gupta, K.K. Chaubey, S. Chakraborty, R. Tiwari, R. Deb and K. Dhama, 2014. Incidence of Mycobacterium Avium Subspecies paratuberculosis in Mehsani and Surti Goats of Indian Origin using Multiple Diagnostic Tests. Journal of Biological Sciences, 14: 124-133.

DOI: 10.3923/jbs.2014.124.133

URL: http://scialert.net/abstract/?doi=jbs.2014.124.133

 
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