Subscribe Now Subscribe Today
Science Alert Home Journals at Science Alert For Authors For Subscribers Contact Us
   
Journal of Biological Sciences
  Year: 2010 | Volume: 10 | Issue: 3 | Page No.: 190-199
DOI: 10.3923/jbs.2010.190.199
Molecular Prediction of Dehalogenase Producing Microorganism using 16S rDNA Analysis of 2,2-dichloropropionate (Dalapon) Degrading Bacterium Isolated from Volcanic Soil
A.A.A. Hamid, S. Hamdan, S.H.Z. Ariffin and F. Huyop

Abstract:
A phylogenetic analysis of an unknown strain AZZ2 isolated from volcanic area Gunung Sibayak Indonesia was performed. Their phylogenetic relationships were analysed using MEGA4 software® to ascertain its evolutionary distance by reconstructing a phylogenetic tree of these organisms. The evolutionary history and bootstrap consensus tree were inferred using the Neighbor-Joining method from 500 replicates. The tree is drawn to scale, with branch lengths (next to the branches) in the same units as those of the evolutionary distances used to infer the phylogenetic tree. The evolutionary distances were computed using the p-distance method and were in the units of the number of base substitutions per site. Based on the partial 16S rDNA sequence determination, the strain showed high sequence similarity to Citrobacter sp. strain JC73/SL7. AZZ2 gene was also compared among known dehalogenase producing bacteria 16S rDNA genes. The results suggested that AZZ2 was closely related to the Serratia marcescens HL1. On the basis of phylogenetic identification only, AZZ2 was subjected to grow on 2,2-dichloropropionate (2,2DCP). The results suggested that strain AZZ2 can degrade 2,2DCP as expected similar to the characteristic of strain HL1 that can grow on halogenated compound. From this study, there was a possibility to predict the phenotype of newly isolated bacteria. The present findings also show that the evolutionary relationships of 16S rDNA gene strain AZZ2 were illustrated by phylograms and both topology are not in good agreement and may suggest an uncertainty of the origin of dehalogenases in volcanic area.
 [Fulltext PDF]   [Fulltext HTML]   [XML: Abstract + References]   [References]   [View Citation]  [Report Citation]
 RELATED ARTICLES:
  •    Characterization and Identification of Bacteria Infecting Indian Tropical Tasar Silkworm, Antheraea mylitta D.
  •    Biodegradation of Low Concentration of Monochloroacetic Acid-Degrading Bacillus sp. TW1 Isolated from Terengganu Water Treatment and Distribution Plant
  •    Molecular Identification and Characterization of a Bacterium that has Potential to Degrade Low Concentration of Haloalkanoic Acid
  •    Biodegradation of Monochloroacetic Acid by a Presumptive Pseudomonas sp. Strain R1 Bacterium Isolated from Malaysian Paddy (Rice) Field
  •    Enzymatic Dehalogenation of 2,2-Dichloropropionic Acid by Locally Isolated Methylobacterium sp. HJ1
  •    Degradation of D,L-2-chloropropionic Acid by Bacterial Dehalogenases that Shows Stereospecificity and its Partial Enzymatic Characteristics
How to cite this article:

A.A.A. Hamid, S. Hamdan, S.H.Z. Ariffin and F. Huyop, 2010. Molecular Prediction of Dehalogenase Producing Microorganism using 16S rDNA Analysis of 2,2-dichloropropionate (Dalapon) Degrading Bacterium Isolated from Volcanic Soil. Journal of Biological Sciences, 10: 190-199.

DOI: 10.3923/jbs.2010.190.199

URL: http://scialert.net/abstract/?doi=jbs.2010.190.199

 
COMMENT ON THIS PAPER
.
 
 
 
 

 

 
 
 
 
 
 
 
 
 

 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 

                 home       |       journals        |       for authors       |       for subscribers       |       asci
          © Science Alert. All Rights Reserved