This study was focused on the quantitative analysis
of Conjugated Linoleic Acids (CLAs) in rumen liquor and assessment of
the relationship between CLAs and other carbon 18 fatty acids and rumen
pH of cattle slaughtered for meat consumption. The fatty acids were extracted
using modified Folch method and determined by gas chromatography. The
mean (n = 22) level of CLAs in the rumen liquor was 14.97 ±7.46
mg/100 mL. Cis-9, trans-11(c9, t11) was the dominant isomer of CLA followed
by trans-10,cis-12 (t10, c12). The level of CLAs was positively correlated
with the concentration of octadecatrienoic (18:3), cis-9, cis-12 octadecadienoic
(18:2), trans-11octadecenoic (18:1) and octadecenoic (18:0) acids. Ruminal
pH was positively correlated with the amount of c9, t11 and total CLA
and negatively correlated with t10, c12 CLA isomer. All these correlations
and relationships indicated that CLAs production in the rumen is dependent
on availability of carbon 18 fatty acids and pH of the rumen liquor.
M.B. Achenef, A.K. Arifah, Y.M. Goh, A.Q. Sazili, O. Fauziah, A. Zuraini, M.N. Somchit and Z.A. Zakaria, 2009. Conjugated Linoleic Acid in Rumen Liquor: Their Relation with Other Carbon 18 Fatty Acids and Rumen pH. Journal of Biological Sciences, 9: 567-572.