In this study, the sensitivity of 100 P. aeruginosa isolates to ciprofloxacin has been investigated by determining minimum bactericidal concentration. The susceptibilities of isolates to other antibiotics were tested using agar disk diffusion method. The isolates contains 67 isolate from urine, 19 isolate from wound, 10 isolate from sputum, two isolate from blood, one isolate from stool and one isolate from ear. Thirty-five percent of isolates showed resistance to 10 antibiotics and also to ciprofloxacin and were sensitive only to imipenem. Thirty-five antibiotypes were recognized for all the isolates. The rates of resistances were determined to antibiotics as follows: gentamicin 49%, ticarcillin 100%, ceftizoxime 78%, co-trimoxazole 97%, amikacin 35%, carbenicillin 65%, ceftriaxone 65%, piperacillin 53%, imipenem 2%, kanamycin 65% and ofloxacin 71%. Fifty-five percent of isolates were resistant to ciprofloxacin. In conclusion, ciprofloxacin-resistant P. aeruginosa has been frequent in our clinical isolates, this data remind the worldwide emerging resistance against ciprofloxacin and this is a serious problem in therapeutic management of P. aeuroginosa infections and has a local and worldwide concern.