The aim of the present study was analyzed the expression of epidermal growth factor receptor (EGFR), matrix metalloproteinase-2 and 9 for evaluating their value as a prognostic markers in transitional bladder cancer specimens. The EGFr, MMP-2 and MMP-9 proteins were assayed semiquantitatively in 85 transitional bladder carcinomas of varying grade and stage. Specific monoclonal antibodies for EGFr, MMP-2 and MMP-9 were used to detect the antigens by immunohistochemical staining of paraffin-embedded tissue sections. Of the 85 specimens, 38 (44.7%) overexpressed EGFr, 38 (41.2%) MMP-2 and 38 (44.7%) MMP-9 proteins when three observers scored stained sections independently. The 5-year disease-specific survival was significantly with EGFr, MMP-2, MMP-9, (p<10-6 for all three markers). In multivariate analysis, the three markers remained the prognositic in bladder cancer and the more significant was EGFr. The results of this study demonstrated that EGFr, MMP-2, MMP-9 proteins overexpression may be an independent prognostic biomarker for bladder cancer.