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Journal of Applied Sciences

Year: 2009 | Volume: 9 | Issue: 18 | Page No.: 3357-3363
Research Article

Evaluation of Commercial Colilert18-Quantitray® Method by ISO Techniques for Enumeration and Quantification of Total Coliforms and Escherichia coli in Drinking-Water of Buraidah, Saudi Arabia

A. Al-Turki
Department of Plant Production and Protection,College of Agriculture and Veterinary Medicine, Qassim University, Buraidah, Saudi Arabia
M.G. El-Ziney
Department of Food Science and Human Nutrition, College of Agriculture and Veterinary Medicine, Qassim University, Buraidah, Saudi Arabia
In the present investigation, the Colilert18-QuantiTray® test is compared with ISO 9308-2:1999 Multiple-Tube Fermentation (MTF) and 9308-1:2000 Membrane Filtration (MF) methods for the detection of coliforms and E. coli in Buraidah drinking water. Regarding sensitivity and specificity of test methods using MTF as reference, the MF method showed a weak sensitivity, while the Colilert18® test showed the highest one. On the other hand, MF method exhibited a higher specificity compared with Colilert18® test. A moderately strong relationship among test methods of coliforms log transformed counts obtained with MF (cfu/100 mL) and Coliert18®/Quanti-Tray (MPN/100 mL) tests compared to MPN-MTF method was demonstrated. Regression analysis revealed the presence of strong linear correlations (p<0.01) between the three test methods and standard plate count of detecting the concentration of serially diluted E. coli LMG 2092 with high regression coefficients with MF and Colilert18® and lower value for MTF. The Colilert18® had E. coli detection down to 5 MPN/100 mL (≅ 0.05/1 mL). Results showed that Coliert18®/Quanti-Tray method is comparable with ISO methods further, the former method has more advantages such as higher sensitivity, maximum detection limit ability and time and labor saving.
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