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International Journal of Zoological Research > Abstract |
International Journal of Zoological Research
Year: 2009 | Volume: 5 | Issue: 4 | Page No.: 161-170
DOI: 10.3923/ijzr.2009.161.170
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| Detection of Apoptotsis Induced by Gentamicin in Rat Hepatocytes |
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Saad Alkahtani,
Saud A. Alarifi
and Amin Abdullah Al-Doaiss
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Abstract:
The aim of the present study was to investigate the effect of antibiotic gentamicin on the apoptotic pathway in rat liver cells. For this purpose, four groups of rats were injected intramuscularly (im) with gentamicin at a dosage of 80 mg kg-1 b.wt. once daily for 1, 2, 3 and 4 weeks successively. The induced apoptosis in hepatocytes was evaluated as early as one day post-injection, the gentamicin started to induce apoptosis as assessed histologically and with the aid of Poly (ADP-ribose) polymerase (PARP) test. Histopathological examination and PARP test revealed relevant apoptosis markers at every weekly interval compared with the negative control. Irregular and condensed nuclear materials, organelles deteriorations and PARP fragmentation were the most alternations. This study demonstrated the potentiality of gentamicin to induce apoptosis in rat liver cells and the usefullness of the obtained data in daily clinical practice regarding the associated development of hepatotoxicity. |
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