Acetone dried pituitary extract obtained from African
bullfrog, Rana adspersa, was stored over a period of 16 weeks and
used to induce spawning in the female Clarias gariepinus. Fresh
pituitary extract was used as control. Fecundity and egg weight decreased
with storage period. Fish injected with pituitary extract stored for 12
weeks and above had significantly (p<0.05) lower % fertilization and
% hatching compared with fish stored for less than 4 weeks. The potency
and viability of R. adspersa pituitary extracts decreased with
storage periods. The results of this study indicated that stored R.
adspersa pituitary hormone extract stored for 4 weeks gave the best
result. Its yield was significantly different from that gotten using hormones
stored for over 8 to 16 weeks period. It showed that the frog pituitary
extract is still potent, viable and effective when stored for not more
than 4 weeks. The R. adspersa pituitary hormone stored for 4 weeks
can therefore be used as optimum storage period under the experimental
conditions used in this study.
O.T. Adebayo and O.A. Fatoyinbo, 2008. Effect of Storage Period on the Efficacy of African Bull Frog Pituitary Extract for Induced Spawning of Clarias gariepinus. International Journal of Zoological Research, 4: 77-80.