Abstract: Amphiprion sebae kept in the recirculating Marine Research Aquarium spawned a maximum of 0.8 times per month throughout the year. This is the first spawning record from a recirculating aquarium environment. Generally the spawning of A. sebae has been described from fresh seawater by various authors. The present study mainly observes the maintenance of brooders, their pairing and spawning in recirculating aquarium environment. Among the 54 adult fishes collected from the natural environment, three pairs are formed in the aquaria. The parental care of egg guarding male was observed. Three pairs are fed with different feed i.e., prawn meat, fish meat and a combination of prawn, fish and clam meats. The spawning frequency was observed to be moderately high (0.8/month) when fed with the mixed feed, whereas a frequency of 0.5 and 0.6 per months was observed when fed with prawn, fish meats respectively. The maximum clutch size (911) was observed also in mixed feeds and it was minimum (395) when the pairs were fed with fish meat alone. The increase in the frequency of spawning was during summer months compared to other months.
Olivia J. Fernando, K. Raja and T. Balasubramanian , 2006. Studies on Spawning in Clownfish Amphiprion sebae with VariousFeed Combinations under Recirculating Aquarium Conditions. International Journal of Zoological Research, 2: 376-381.