Abstract:
Experiments were conducted
to compare the ability of a medium supplemented with organic acids to
the ability of commercially available, non-selective media to support
growth of Campylobacter spp. and Arcobacter butzleri.
Liquid medium was composed of yeast extract-peptone basal broth (BB)
that could be supplemented with an organic acid (OA) mixture of fumaric,
lactic, malic, and succinic acids (BB + OA). Growth of cultures in BB
and in BB + OA was compared to growth in Brucella broth, Mueller-Hinton
broth, and Fluid Thioglycollate medium. Growth of A. butzleri and
10 of 15 Campylobacter isolates was significantly (P <
0.05) greater in BB + OA than in other broth media, while there was
no significant difference in growth of the other 5 Campylobacter
isolates in BB, BB + OA, Brucella broth, Mueller-Hinton broth, or Fluid
Thioglycollate medium. Growth was also compared on agar media composed
of BB + OA with Bacto agar and hemin (BA-hemin + OA) and on Remel® Blood
Agar. Results indicated that there was no significant difference in
the number of cfu`s recovered on BA-hemin + OA and on blood agar. Findings
indicate that media supplemented with organic acids may be used as alternatives
to some currently available commercial media for growing and maintaining
Campylobacteriaceae.
Arthur Hinton and Jr. , 2006. Comparison of Growth of Campylobacteriaceae on
Media Supplemented with Organic Acids and on Commercially Available
Media . International Journal of Poultry Science, 5: 99-103.