A total of 720 individuals
of 12 indigenous chicken populations, geographically localized in Southern
China were genotyped for 30 microsatellite markers in polymerase chain
reaction (PCR) to evaluate the genetic variation and genetic distance
between populations. All microsatellites were found to be polymorphic.
Heterozygosity was calculated to determine the genetic variation. Of
the 30 microsatellite loci, number of alleles per locus (Na) and effective
number of alleles per locus (Ne) ranged from 4 to 11 and 2.157 to 8.019,
respectively. The average expected heterozygosity (HE) was
0.669, while the average observed heterozygosity (HO) was
0.764. The polymorphism information content (PIC) has values between
0.560 and 0.641. Using Nei`s standard distance, genetic distance (DA)
calculated ranged between 0.088 (Guanxi Sanhuang vs. Nandan Yao) and
0.495 (Huiyang Beard vs. Zhangzhou Game). The topology of phylogenetic
trees constructed showed general patterns of relationship and genetic
differentiation among the indigenous populations studied, however, both
trees from Neighbor-Joining method and Unweighted Pair Group method
showed a similar topology. The results provided evidence of the applicability
of microsatellite to determining the genetic relatedness among different
Chinese indigenous chicken populations and evaluating of genetic variations.
Yu Ya-Bo , Wang Jin-Yu , D.M. Mekki , Tang Qing-Ping , Li Hui-Fang , Gu Rong , Ge Qing-Lian , Zhu Wen-Qi and Chen Kuan-Wei , 2006. Evaluation of Genetic Diversity and Genetic Distance
Between Twelve Chinese Indigenous Chicken Breeds Based on Microsatellite
Markers. International Journal of Poultry Science, 5: 550-556.