A total number of five plants
were tested qualitatively to the presence of microbicidal activity by
Kirby-Bour method in agar gel plates using poultry pathogenic E.
coli as microbes. Among the five herbs used two herbs namely Piper
betal and Cassia auriculata found to have microbicidal activity.
The potency of microbicidal activity of that two herbs were tested quantitatively
by the same method by using different dilutions and in different solvents,
clinical experiments conducted in birds with infection and found that
the plant Cassia auriculata have had more potent microbicidal
activity when compare to Piper betel and screened for side effects,
additional effects in birds.