In this study, anti-bacterial activity of aqueous extract of P. igniarius against selected gram positive and gram negative bacteria using agar diffusion, broth macrodilution and agar dilution methods were investigated. The agar diffusion method revealed that P. igniarius aqueous extract showed inhibition zone against only S. aureus ATCC 24923. The susceptibility test against MSSA and MRSA revealed that P. igniarius aqueous extract showed inhibition zone against all of MSSA strains but cannot inhibit growth of MRSA. The broth macro dilution and agar dilution methods reveal MIC and MBC of P. igniarius against S. aureus ATCC 25923 was 1.25 and 2.5 g L-, respectively while MICs and MBCs against MSSA were 1.25-2.5 g L- and 0.25-0.5 g L-, respectively. The aqueous extract of P. igniarius showed inhibitory effect on growth of MSSA but not MRSA.