Antioxidant Activity of Aqueous Methanol and Ethyl Acetate Extract of Leaves of Annona senegalensis Pers from Togo Versus the One Originates from Burkina Faso
Abstract:
The aim of the present study is to evaluate and compare
the antioxidant potential of the leaves extracts of Annona senegalensis
(Annonaceae) of Togo versus the one of Burkina Faso. To this end, aqueous
methanol and ethyl acetate extracts by splitting and by steeping were
achieved and the determination of total polyphenols of which flavonoids
was carried out. A survey of the antioxidant activity using the DPPH methods
was performed. The content in total polyphenol (3.47 ± 0.03%) and
flavonoid (2.33 ± 0.17%) of 70% (v/v) aqueous methanol extract
of the specimen from Togo was significantly higher than the one from Burkina
(2.66 ± 0.08 and 1.64 ± 0.04%, respectively) (p<0.00001
for total polyphenol; p<0.05 for total flavonoid), whereas, the amount
of total flavonoid in the ethyl acetate extract of the species from Burkina
(40.38%) was triplicated. For the two types of extracts, the species of
Burkina Faso showed an improved antioxidant activity than the one of Togo
(IC50 = 8.51 and 21.08 μg mL-1 versus 12.46
and 29.22 μg mL-1, respectively) (p<0.05). These free
radicals inhibition activity of the extracts may be due at least to polyphenolic
flavonoids identified by means of HPLC assay performed in the preliminary
study. These flavonoids were rutin and isoquercetrin as flavanols (specimen
from Togo) of which are added epicatechin and catechin derivatives (flavanols)
in the specimen from Burkina. The traditional use of plant leaves may
imply in part this activity against the free radicals.
Y. Potchoo, I.P. Guissou, M. Lompo, E. Sakie and B. Yaro, 2008. Antioxidant Activity of Aqueous Methanol and Ethyl Acetate Extract of Leaves of Annona senegalensis Pers from Togo Versus the One Originates from Burkina Faso. International Journal of Pharmacology, 4: 120-124.
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