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International Journal of Cancer Research
  Year: 2009 | Volume: 5 | Issue: 3 | Page No.: 123-129
DOI: 10.3923/ijcr.2009.123.129
In vitro Cytotoxic and Apoptotic Properties of the Stem Bark Extract of Sandoricum koetjape on Breast Cancer Cells
A.F.A. Aisha, S.A. Alrokayan, K.M. Abu-Salah, Y. Darwis and A.M.S. Abdul Majid

Abstract:
Both cytotoxic and apoptotic properties of the stem bark extract of Sandoricum koetjape were investigated in vitro. 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium- 5-carboxanilide (XTT) cell proliferation assay was used to study the cytotoxic properties on three breast cancer cell lines and one normal cell line; MCF-7, MDA-MB-231, T47D and MCF-10A, respectively. Caspase Glo 3/7 assay was used to study the apoptotic activity on MCF-7 as a model cell line. The n-hexane extract showed a dose dependent growth inhibition of all tested cell lines with IC50 values between 44 and 48 μg mL-1. At 100 μg mL-1, the extract induced apoptotic cell death of MCF-7 by inducing activity of the effector caspases 3 and 7. These results indicated that n-hexane extract of S. koetjape has cytotoxic and apoptotic properties on breast cancer cell lines making it a good candidate for further studies.
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How to cite this article:

A.F.A. Aisha, S.A. Alrokayan, K.M. Abu-Salah, Y. Darwis and A.M.S. Abdul Majid, 2009. In vitro Cytotoxic and Apoptotic Properties of the Stem Bark Extract of Sandoricum koetjape on Breast Cancer Cells. International Journal of Cancer Research, 5: 123-129.

DOI: 10.3923/ijcr.2009.123.129

URL: http://scialert.net/abstract/?doi=ijcr.2009.123.129

 
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