This study aimed at the evaluation of sensitivity and reproducibility of the modified o-phthalaldehyde colorimetric method in determination of cholesterol in the Lactobacillus specific laboratory media; (de-Man Rogosa Sharpe medium). The results indicated that at the range of 0-100 mg L-1 cholesterol concentration, the quantity of measured cholesterol was affected by the type of cholesterol. Application of the method for water soluble cholesterol yielded less quantity of cholesterol in comparison to the same situation for free alcoholic cholesterol. Also, the method did not present substantial reproducibility. Therefore, cholesterol containing MRS solutions cannot be accurately distinguished using this method.
Maryam Mirlohi, Golnoosh Madany, Akabar Hassanzadeh and Mohamad Javad Yahay, 2012. On the Colorimetric Method for Cholesterol Determination in the Laboratory Media. International Journal of Biological Chemistry, 6: 37-41.