Wheat anther culture produces homozygous doubled haploid plants, in a very
short time span as comparing with traditional breeding methods. It is affected
with several different factors, such as suitable spike stage as a donor for
anthers, anthers cold pretreatment duration, rate of embryogenic callus formation
and the time of anthers transferring from callus induction to regeneration medium.
The effects of these factors on androgenic process were studied in this work
the response of five F1 wheat hybrids, of ten varieties, differing
in their salt tolerance, was investigated in anther culture system for haploid
plantlets regeneration. Anthers were plated on P4S medium for callus induction,
anthers were taken from different spikes ages (as in a distance between the
top of the spike and flag leaf), different duration of cold pretreatment and
different time of transferring anthers from callus induction to regeneration
medium (190-2) were studied. Significant differences between genotypes on androgenic
process were detected. Genotype influence was significant on the suitable spike
length which has high percentage of proper stage of mid-uninucleate stage, consequently
on both of callus induction and plant regeneration (green, albino and total
plantlets). On the other hand, there was no significant influence in the embryogenic
callus formation per 100 anthers between the hybrids under same duration (14,
28 and 56 days). The suitable duration for staying on induction callus medium
was 28 days as the green regeneration plants increased. This is considered as
accurate parameter for evaluation efficiency of androgensis for anther culture.
Aida A. Rizkalla, A.M.F. Al-Ansary, S.A.A. Attia, A.A.A. Haiba and J.E. Nasseef, 2012. Response of Some Egyptian and Introduced Wheat Hybrids to Androgenic Process. International Journal of Agricultural Research, 7: 205-214.