Abstract:
Molecular and morphological analyses were used to study
phenotypic and genotypic characteristics of two common sheep and camel
strains of Echinococcusgranulosus. Biometric analysis of
fifteen characters showed that those 15 morphometric values of camel isolates
have significantly higher than sheep isolates. Number of hooks, length
and width total of the handle, the blade and the distance between the
blade and the guard of large and small hooks were those characters that
measured. Molecular analysis of ND1 fragment of mtDNA obtained from G1
and G6 genotypes using PCR-RFLP technique with two commercial restriction
endonucleases enzyme AatI and BstHHI were in agreement with the morphological
findings. PCR-RFLP band patterns using AatI demonstrated two bands of
170 and 301 bp in sheep strain whereas camel strain remained undigested.
Besides PCR-RFLP profiles using BstHHI demonstrated two bands of 137 and
334 bp identical to the G6 genotype and one DNA fragment of 471 bp in
G1 genotype. Because of the intraspecific variation of sheep and camel
strains using both morphological and molecular tools concomitantly provides
more accurate and reliable information about the phenotypic and genotypic
characteristics of E. granulosus isolates and emphasizes the valuable
tools in transmission and epidemiological studies.
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