Abstract:
Soil samples were collected from different states in
Southern India to isolate and characterize actinomycetes at molecular
levels through plasmid DNA and protein pattern. A total of 12 soil samples
were collected from which four strains (AP-1, KN-2, KL-3 and TN-4) were
isolated and characterized as Streptomyces sannanensis. The antimicrobial
activity of these strains was studied against gram-negative and positive-bacteria.
It was capable of producing antibiotic against gram-positive, while gram-negative
bacteria were not affected. The potential of antibiotic production of
these strains is likely to be chromosomally encoded by confirming the
detection of plasmid DNA. The strains such as KN-2 and KL-3 showed two
plasmids and the other two strains showed only one. This is a preliminary
step to correlate the chemotaxonomic relationship among the strains of
Streptomyces spp. for secondary metabolite production.
P. Ponmurugan and B. Nithya, 2008. Plasmid DNA of Antibiotic Producing Strains of Streptomyces sannanensis Isolated from Different States in Southern India. Biotechnology, 7: 487-492.
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