In the present study plant regeneration techniques of Aegle marmelos from nodal explant have been developed. For inducing callus, nodal segments derived from in vitrogrown shoots were cultured on Murashige and Skoog (MS) medium supplemented with different compositions of phytohormones. The maximum callus formation was observed on MS medium having 0.3 mg L-1 BA+2.0 mg L-1 2,4-D. In all cases, callus proliferation began at the cut ends of the explants. Optimum organogenesis and plantlet formation occurred when the calli were subcultured on medium fortified with 2.0 mg L-1 l BA+0.1 mg L-1 NAA. Callus-derived shoots produced roots when transferred to half strength of MS medium without any growth regulators. The rooted plantlets were successfully transplanted to soil.