Four different media namely- Hyponex, Murashige and Skoog (MS), OKF1 and Knudson C (KC), were tested for large scale multiplication of sympodial native orchid, Dendrobium transparens Wall. via seeds. MS medium was found to be best for characters studied in Dendrobium transparens, followed by Hyponex medium but OKF1 medium gave the least performance. Days required to seed germination was the minimum (50 days) in MS medium while the maximum days (59 days) was required by OKF1 medium. Considering other characteristics, such as days required to protocorm formation and plantlet development, number of leaves and roots per plantlet, plantlet height and root length and finally plant survivability percentage, MS medium showed significantly better performance for in vitro seed propagation of Dendrobium transparens.
M.K. Alam, M.H. Rashid, M.S. Hossain, M.A. Salam and M.A. Rouf, 2002. In vitro Seed Propagation of Dendrobium (Dendrobium transparens) Orchid as Influenced by Different Media. Biotechnology, 1: 111-115.