Abstract:
An experiment was designed to standardize the protocol for anther culture and to identify the best rice line and hybrid suited for androgenesis. The hybrids IR 58025 A x IR 66 R and IR 58025 x C20R, two Assam cultures, ARC 15759, ARC 18023, one japonica type Taipei 309 and best performing local variety ASD16 was used for anther culture studies. The hybrid, IR 58025A x IR 66R, responded well and produced more per cent calli in 2.5 mg L-1 of 2, 4D +3 mg L-1 of kinetin. The combination of 2.5 mg L-1 of 2, 4D+3 mg L-1 of kinetin was observed earlier for calli induction when compared to the other combination. The hybrid, IR 58025A x IR 66R was best hybrid for anther culture studies. The combination of 2.5 mg L-1 of 2, 4D+3 mg L-1 of kinetin was observed earlier for calli induction when compared to other combination. Among the parents, ARC 18023 and ASD 16 found to be anther culture friendly lines.
S. Hari Ramakrishnan, S. Saravanan , C.R. Anandakumar and J.R. Kannanbapu , 2005. In vitro Androgenesis in Rice (Oryza sativa L.). Asian Journal of Plant Sciences, 4: 600-602.
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