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Asian Journal of Plant Pathology
  Year: 2013 | Volume: 7 | Issue: 1 | Page No.: 42-53
DOI: 10.3923/ajppaj.2013.42.53
In vitro Antagonistic Potential of Fungal Isolates Against Botrytis fabae Sard
Ermias Teshome, Chemeda Fininsa and Samuel Sahile

Abstract:
Owing to its high protein content, faba bean (Vicia fabae L.) leaves’ phylloplane harbors many microorganisms besides Botrytis fabae which could have antagonistic potential. The use of chemical fungicides against chocolate spot has been a common practice, but negative effects to the environment forces a search for alternative options. The objective of this study was to explore fungal isolates residing on faba bean leaves and evaluate their antagonistic potential against B. fabae. For this matter, 236 leaf samples were collected from different districts of West Hararghe and Bale zones, which yielded 72 fungal isolates. These isolates were evaluated for their biocontrol potential against B. fabae in vitro. ANOVA (Analysis of Variance) for Antibiosis, percent growth inhibition, growth rate and parasitism tests showed significant difference (p<0.0001) among fungal isolates. In antibiosis test, two isolates, Trichoderma harzianum and Penicillium spp. formed higher mean inhibition zone of 7.33 and 7.00 mm, respectively and the highest mean colony growth inhibition were recorded from T. harzianum (69%) and T. oblongisporum (58.1%) over the control. Trichoderma spp. showed higher mean growth rate (14-16.67 mm day-1) than any of other fungal isolates and on the other hand higher mean diameter of lysed mycelium (12 mm) was recorded from Aspergillus spp. and Penicillium spp. This study revealed higher distribution of biocontrol agents and their antagonistic ability over B. fabae, in Ethiopia. Particularly Trichoderma spp. showed better potential in controlling B. fabae and can be further evaluated for its commercialization, either alone or as a component of integrated disease management.
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How to cite this article:

Ermias Teshome, Chemeda Fininsa and Samuel Sahile, 2013. In vitro Antagonistic Potential of Fungal Isolates Against Botrytis fabae Sard. Asian Journal of Plant Pathology, 7: 42-53.

DOI: 10.3923/ajppaj.2013.42.53

URL: http://scialert.net/abstract/?doi=ajppaj.2013.42.53

 
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