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Asian Journal of Plant Pathology
  Year: 2011 | Volume: 5 | Issue: 4 | Page No.: 146-154
DOI: 10.3923/ajppaj.2011.146.154
First Report of Diaporthe/Phomopsis Complex Isolates in Soybean from Malaysia and Their Longevity in Stored Seeds
Samiyeh Raeisi, Adam Puteh and Kameruzaman Sijam

Abstract:
Diaporthe/Phomopsis Complex (DPC) can infect soybean seed and reduce its germination in the field and also survive on the seed during storage. Ten soybean seed lots which were stored at 0°C for up to 13 years were used to evaluate the percentage of DPC infection on them and also to identify the DPC isolates. Morphological and molecular methods were used to determine the longevity and frequency of DPC isolates in stored soybeans seeds. Conventional and nested Polymerase Chain Reaction (PCR) was used for amplification of pure cultures and seed lots. The longevity of isolates in storage was found to be <9 years which suggested that Diaporthe/Phomosis sp., can survive up to 9 years in cold storage (0°C). Six isolates of DPC were detected, identified and characterized based on morphological and molecular methods in soybean seeds for the first time in Malaysia. Most of the isolates identified belonged to Phomopsis longicolla and only one isolate identified from Diaporthe Phaseolorum var. sojae. All isolates that were identified using morphological technique were confirmed using molecular method and registered in national center of biotechnology information (NCBI). The result of this study showed that DPC isolates can survive for long time in storage.
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How to cite this article:

Samiyeh Raeisi, Adam Puteh and Kameruzaman Sijam, 2011. First Report of Diaporthe/Phomopsis Complex Isolates in Soybean from Malaysia and Their Longevity in Stored Seeds. Asian Journal of Plant Pathology, 5: 146-154.

DOI: 10.3923/ajppaj.2011.146.154

URL: http://scialert.net/abstract/?doi=ajppaj.2011.146.154

COMMENTS
13 December, 2011
k. sridhar:
my Msc Ag thesis topic is study on phomopsis blight of Brinjal in Raipur 1996 Chattisgarh India related to seed pathology work, so the topic may be very useful for me. Best regards K.sridhar Assistant professor Plantpathology TNAU Affilated vellore
 
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