India has a diversity of medicinal plants. Most of them are used by the villagers against various diseases and are not revealed completely because of the secrecy maintained by them. Ormocarpum cochinchinense is found to be one among them which potentially shows good effect against bone fracture healing. The level of calcium in the plant is found to be one of the reasons for the bone healing effect. The scientific evidence of minerals present in the plant was important to prove the health effect of such medicinal plant. Since, the availability of O. cochinchinense is rare, an approach was made to study under in vitro condition. Callus induction was fast and reliable with Murashige and Skoog media containing 3 mg L-1 of kinetin and 1 mg L-1 of 2 4-D. The extract from leaves and callus was subjected to biomeneralization for calcite growth. The obtained calcite crystals were analysed with scanning electron microscope and the levels of calcium was quantified. Individual crystals were rod shaped and hexagonal in transactional view. When comparing the levels of minerals in the leaf and callus extract, it clearly indicated that the amount was high with leaf extract, but the variation was less. Results proved that the tissue culture of O. cochinchinense can be an alternative for biomeneralization of calcite crystals and also it can be used as a source for bone healing aid. The property of the plant in bone healing has to be ascertained by further experiments on animal models.
K.M. Maria John, Deepavenkatesan Venkatesan, S. Sandhiya, S. Karthik and Sampath Natarajan, 2011. In vitro Synthesis of Calcite Crystals from Ormocarpum cochinchinense (L.) a Traditional Bone Healing Aid of Southern India. American Journal of Plant Physiology, 6: 312-317.