Grapes (Vitis viniferaL.) are believed to have health benefits due to their antioxidant activity and polyphenols. Thus scientists have conducted research to explore their positive effects on many chronic diseases. However, the phenolic content is variable depending on grape variety. In this study, we examined the grape hybrids total phenolics and antioxidant activities during the two different harvesting times in 2008 and 2009. Total phenolic content and antioxidant activity of hybrids were significant among hybrids for both years. However, the relationship between different harvest times was not significant. In 2008 KXP-10 hybrid and in 2009 BX2-149 hybrid have shown a higher phenolic and antioxidant activity according to the other varieties. These hybrids have dark berry color and seed. In 2009, hybrids which have seed total phenolic content and antioxidant activity were investigated with seed and without seed. Greater value was obtained hybrids which have analyzed with seed. Before registration 23 different hybrids or variety candidates which were obtained different Institutes were determined total phenolic content and antioxidant content.